Public Class Form1

    Private Sub Button1_Click(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles Button1.Click
        'Create streamreader and open text file
        Dim sr As IO.StreamReader = My.Computer.FileSystem.OpenTextFileReader("C:\testfile.txt")
        TextBox1.Text &= sr.ReadLine & vbCrLf
        TextBox1.Text &= sr.ReadLine & vbCrLf
        'Don't close the streamreader
        'sr.Close()

    End Sub

    Private Sub Button2_Click(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles Button2.Click
        Dim sr As IO.StreamReader = My.Computer.FileSystem.OpenTextFileReader("C:\testfile.txt")
        TextBox1.Text &= sr.ReadLine & vbCrLf
        TextBox1.Text &= sr.ReadLine & vbCrLf

    End Sub
End Class

Imports System.IO

Public Class Form1
	Private filename As String = "C:\test\testfile.txt"
	Private IntegerList As List(Of Integer)
	Private CurStream As StreamReader

	Private Sub Button1_Click(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles Button1.Click
		CurStream = New StreamReader(filename)

	End Sub

	Private Sub Button2_Click(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles Button2.Click

		Dim CurRecord As String
		Dim strSplit() As String
		CurRecord = CurStream.ReadLine()
		If CurRecord Is Nothing Then
			MsgBox("No more records available")
		Else
			strSplit = CurRecord.Split(",")
			TextBox1.Text = strSplit(0)
			TextBox2.Text = strSplit(1)
			TextBox3.Text = strSplit(2)
			TextBox4.Text = strSplit(3)
		End If
	End Sub
End Class